rabbit cell signalling technology 9315 p ser9 gsk 3β elisa capture  (Cell Signaling Technology Inc)

Journal: Journal of Medical Virology

Article Title: Clinical, Serological, and Molecular Profile of Dengue Patients With Warning Signs During the 2024 Outbreak in Belo Horizonte, Brazil

doi: 10.1002/jmv.70805

Figure Lengend Snippet: Molecular and serological characteristics of hospitalized dengue patients. Serum samples collected at hospital admission were tested by RT‐qPCR for DENV (serotypes 1–4), CHIKV, ZIKV, MAYV, and OROV, and by ELISA for DENV‐specific IgM and IgG. (A) Proportion of patients testing positive for DENV, CHIKV, and DENV/CHIKV coinfection, as well as RT‐qPCR–negative cases. (B) Distribution of DENV serotypes among RT‐qPCR–positive patients, highlighting the predominance of DENV‐1 and the detection of DENV‐2, DENV‐3, and mixed infections (DENV‐1/DENV‐2 and DENV‐1/DENV‐3). No DENV‐4 cases were identified. (C) Serological profiles based on IgM and IgG ELISA results, including single‐ and dual‐antibody positivity and their relationship with RT‐qPCR outcomes, illustrating partial overlap between molecular and serological diagnostic windows.

Article Snippet: For serological evaluation, ELISA was performed on patient sera using the PanbioTM Dengue IgM Capture ELISA kit (Abbott, EUA) for IgM detection and the Euroimmun Dengue Virus IgG ELISA kit (Euroimmun, Germany) for IgG detection.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Diagnostic Assay

Journal: Cell Communication and Signaling : CCS

Article Title: The NewroBus platform: engineered humanized anti-TfR1 nanobodies for efficient brain delivery

doi: 10.1186/s12964-025-02605-1

Figure Lengend Snippet: Fusion to TfR1b-Nb enables CNS delivery of TNFI-Nb1 via human TfR1-mediated transcytosis. a TNFα-based ELISA quantification of TNFI-Nb1 in serum and brain homogenates from Tfr1 h/w and Tfr1 w/w rats, 6 h after intravenous injection. TNFI-Nb1 was detectable in serum but absent in brain tissue, indicating lack of BBB permeability. b SDS-PAGE and SEC-HPLC analysis of the heterodimer composed of TNFI-Nb1 fused to TfR1b-Nb 04B05R3 (Family A), produced in CHO-S cells. C ) TNFα-based ELISA quantification of TNFI-Nb1 ~ 04B05R3 in serum, CSF, and brain homogenates of Tfr1 h/w and Tfr1 w/w rats, 48 h post-injection. The heterodimer was present in serum of both genotypes but detected in CSF and brain only in Tfr1 h/w rats, demonstrating human TfR1-dependent BBB transcytosis. The observed CSF/serum ratio (TNFα-based ELISA) of ~ 1 indicates efficient CNS delivery. Statistical analyses were conducted using unpaired two-tailed t-tests, with p-values reported in the figure panels. Data are presented as mean ± SEM

Article Snippet: Biotinylated TNFα , ELISA capture antigen , Acro Biosystems , TNA-H82E3-25ug.

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Permeability, SDS Page, Produced, Two Tailed Test